2026-09-20T11:42:09Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/83530 2023-07-11 col_2142_5131 col_2142_14771 com_2142_5130 com_2142_685 com_2142_397

Wheeler, Matthew B. White, Brett Rodger 2015-09-25T21:07:58Z 2015-09-25T21:07:58Z 10000-01-01 1997 1997 Results from embryo culture experiments indicated: (1) bovine serum albumin (BSA) supplementation improved developmental rates of four- and eight-cell Meishan embryos to the blastocyst stage, (2) fetal bovine serum supplementation improved hatching rates of later stage Meishan embryos, (3) fatty acid-free BSA did not improve developmental rates of early stage embryos, (4) more Meishan than Yorkshire one-cell embryos developed to the eight-cell and compact morula stages following 96 hours of culture, (5) more Yorkshire than Meishan two-cell embryos developed to the eight-cell stage following 96 hours of culture and (6) modified Whitten's medium +1.5% BSA was the most efficient medium for culture of one-cell embryos to the blastocyst stage. Results from activation and culture of in vivo matured oocytes indicated: (1) ethanol had no effect on activation rates, (2) cold shock, sham enucleation, electroactivation and electroactivation + culture with cycloheximide improved activation rates, and (3) electroactivation + culture with cycloheximide was the most efficient treatment for production of pronuclear stage cytoplasm. Results from NT experiments indicated that neither enucleated, unactivated oocytes nor enucleated, activated oocytes promoted development of NT embryos constructed from porcine ES cells beyond the four-cell stage. However, enucleated zygotes supported development of NT embryos beyond the four-cell stage to the compact morula stage. These results provide preliminary evidence for in vitro development of NT embryos produced from porcine ES cells. Made available in DSpace on 2015-09-25T21:07:58Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3017009.pdf: 6765256 bytes, checksum: 53d21c1de6637faa12a482e2353a76b3 (MD5) Previous issue date: 1997 Embargo set by: Seth Robbins for item 84811 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 124 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1997. http://hdl.handle.net/2142/83530 (MiAaPQ)AAI3017009 eng Biology, Animal Physiology Nuclear Transfer With Porcine Embryonic Stem Cells text Animal Sciences Animal Sciences University of Illinois at Urbana-Champaign Dissertation Ph.D.