2026-09-18T20:37:07Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/83533 2023-07-11 col_2142_5131 col_2142_14771 com_2142_5130 com_2142_685 com_2142_397

Gaskins, H. Rex McCracken, Vance J. 2015-09-25T21:08:00Z 2015-09-25T21:08:00Z 10000-01-01 2001 2001 The potential for inflammatory cytokines to sensitize intestinal epithelial cells to indigenous bacteria was evaluated in HT-29 cells associated with Lactobacillus plantarum 299v in the presence or absence of tumor necrosis factor-alpha (TNF-alpha). Co-culture of TNF-alpha-stimulated HT-29 cells with L. plantarum 299v increased interleukin-8. (IL-8) mRNA expression above levels observed for cells treated with TNF-alpha alone. The ability of TNF-alpha-to upregulate CD14 and Toll-like receptor (TLR) expression and thereby enhance IL-8 mRNA expression in response to L. plantarum 299v was investigated. Baseline expression of CD14 mRNA and cell-surface CD14 was not altered by TNF-alpha. HT-29 cells did not express TLR2 mRNA. However TNF-alpha increased baseline HT-29 expression of TLR4 mRNA, and co-culture with L. plantarum 299v further enhanced TLR4 mRNA expression in TNF-alpha-treated HT-29 cells. These results indicate that neither CD 14 nor TLRs 2 and 4 are involved in the IL-8 mRNA response by HT-29 cells to L. plantarum 299v. However, these results indicate that intestinal epithelial cell responses to indigenous bacteria may contribute to intestinal inflammation. Made available in DSpace on 2015-09-25T21:08:00Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3017162.pdf: 11668313 bytes, checksum: 4f8d7ee3068a0547f037c55d7a1ad7d3 (MD5) Previous issue date: 2001 Embargo set by: Seth Robbins for item 84814 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 209 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2001. http://hdl.handle.net/2142/83533 (MiAaPQ)AAI3017162 eng Health Sciences, Immunology Intestinal Inflammatory Responses to the Indigenous Microbiota text Animal Sciences Animal Sciences University of Illinois at Urbana-Champaign Dissertation Ph.D.