2026-09-21T15:26:35Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/83587 2023-07-11 col_2142_5131 col_2142_14771 com_2142_5130 com_2142_685 com_2142_397

To further study the intracellular redox environment, two novel technologies were developed: first, a conductive substrate cell culture system to control the intracellular redox environment via electrical potentials; and second, a FRET (fluorescence resonance energy transfer)-based redox biosensor to monitor changes in the intracellular redox environment. Observations of altered intracellular redox status of Chinese hamster ovary (CHO) cells treated with electrical potentials indicate that electrical potentials may be a useful technology for controlling the intracellular redox environment. A rationally-designed FRET biosensor expressed in CHO cells was used for reporting changes related to cell growth. These studies advance the understanding of how the intracellular redox environment controls cell fate and provide new methodologies to study redox regulation of cell fate. Gaskins, H. Rex Conour, John Eugene 2015-09-25T21:08:18Z 2015-09-25T21:08:18Z 10000-01-01 2005 2005 Made available in DSpace on 2015-09-25T21:08:18Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3202079.pdf: 5027055 bytes, checksum: a94c8fcb6354db4acbc2c51dcd74bdfb (MD5) Previous issue date: 2005 Embargo set by: Seth Robbins for item 84868 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 192 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005. http://hdl.handle.net/2142/83587 (MiAaPQ)AAI3202079 eng Biology, Cell Redox Regulation of Cell Fate text Animal Sciences Animal Sciences University of Illinois at Urbana-Champaign Dissertation Ph.D.