2026-09-21T22:11:22Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/83611 2023-07-11 col_2142_5131 col_2142_14771 com_2142_5130 com_2142_685 com_2142_397

Beever, Jonathan E. Meyers, Stacey N. 2015-09-25T21:08:26Z 2015-09-25T21:08:26Z 10000-01-01 2007 2007 Improvement of meat quality is important for consumer satisfaction. As many pork quality traits cannot be assessed in the live animal, selection for these traits must rely upon genetic determinants known as quantitative trait loci (QTL). To facilitate fine-mapping of swine QTL, a ∼1 Mb-resolution human-pig comparative map, composed of 2,274 markers in 34 linkage groups, was constructed using the radiation hybrid method. Comparative analyses revealed 51 conserved synteny groups, including 173 conserved segments, and overall comparative coverage greater than 90%. This map was used, together with porcine linkage and bacterial artificial chromosome (BAC) physical maps, to fine-map a QTL affecting pork tenderness that was detected, on the q arm of porcine chromosome 2 (SSC2q), within the Illinois Meat Quality Pedigree (IMQP). Nine novel microsatellite markers within the ∼30-Mb region surrounding the most likely QTL position, including one within the candidate gene calpastatin (CAST), were developed. These markers, along with nine publicly-available markers, were used to perform linkage and haplotype analyses within the IMQP. Within-family linkage analyses revealed at least two families segregating for a highly-significant QTL in strong positional agreement with CAST. A combined analysis of these two families yielded QTL intervals of 36 cM and 7 cM for Instron shear force and taste panel tenderness, respectively, while haplotype analyses suggested further refinement to a 1.8 cM interval containing CAST. To identify allelic variation that may influence pork tenderness, the complete genomic sequence of porcine CAST was determined. Nearly 77.6% of this gene was then re-sequenced from each of six IMQP F1 boars. Based on heterozygosity, 393 of 896 discovered polymorphisms appeared concordant with previous QTL data. The location of this variation within the CAST gene suggests that a causative mutation is likely to be regulatory. Functional characterization of CAST variation should enhance understanding of the molecular basis of pork tenderness, and thus allow for genetic improvement of pork products. The effectiveness of CAST polymorphisms for marker-assisted selection (MAS) of pork tenderness can now be assessed. Furthermore, genomic resources developed in this study may be used to investigate many other porcine QTL, including additional tenderness QTL suggested on SSC2q. Made available in DSpace on 2015-09-25T21:08:26Z (GMT). No. of bitstreams: 3 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3301269.pdf: 3730024 bytes, checksum: 792cc64c78d3c7298d380e04a9491c59 (MD5) 3301269.zip: 994294 bytes, checksum: e356f5bcbaf639ddab1b2b864d5b90b8 (MD5) Previous issue date: 2007 Embargo set by: Seth Robbins for item 84892 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 134 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2007. http://hdl.handle.net/2142/83611 (MiAaPQ)AAI3301269 eng Agriculture, Animal Culture and Nutrition QTL Mapping and Molecular Dissection of Meat Quality Traits in Swine text Animal Sciences Animal Sciences University of Illinois at Urbana-Champaign Dissertation Ph.D.