2026-09-22T09:04:57Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/83704 2023-07-11 col_2142_5131 col_2142_14781 com_2142_5130 com_2142_687 com_2142_397

Nakamura, Manabu T. Li, Yue 2015-09-25T21:10:07Z 2015-09-25T21:10:07Z 10000-01-01 2006 2006 The aim of the subsequent study was to identify a PPARalpha ligand under EFA deficiency. Lysophospholipids (lysoPL) are the most likely candidates for the ligands because decreased HUFA under EFA deficiency may shift the equilibrium of phospholipid deacylation/reacylation cycle toward accumulation of lysoPL. To test the hypothesis, first we used computer simulation to estimate affinity between lysoPL and PPARalpha. The model predicted lysoPL binding with high affinity. Next, binding affinity of lysoPL to PPARalpha was measured in vitro using a fluorescent ligand displacement assay. The dissociation constants (Kd) of lysoPL were low nanomolar suggesting that they are potent ligands for PPARalpha. Finally, when CV-1 cells were treated with lysoPL, a reporter plasmid with PPARalpha response elements was induced. In conclusion, our study suggests that PPARalpha, together with SREBP-1c, senses HUFA status and confers pathway specific induction of HUFA synthesis by EFA deficient diets. LysoPL may act as signaling molecule and activate PPARalpha in response to essential fatty acid deficiency. Made available in DSpace on 2015-09-25T21:10:07Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3242920.pdf: 2584989 bytes, checksum: fccf99d9389d0e5d260bb8b3ca01cd4e (MD5) Previous issue date: 2006 Embargo set by: Seth Robbins for item 84985 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 94 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2006. http://hdl.handle.net/2142/83704 (MiAaPQ)AAI3242920 eng Health Sciences, Nutrition Peroxisome Proliferator -Activated Receptor (Ppar)alpha Regulation of Highly Unsaturated Fatty Acid Synthesis text Food Science and Human Nutrition Food Science and Human Nutrition University of Illinois at Urbana-Champaign Dissertation Ph.D.