2026-09-22T07:12:28Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/83738 2023-07-11 col_2142_5131 col_2142_14781 com_2142_5130 com_2142_687 com_2142_397

Hans P. Blaschek Chen, Chih-Kuang 2015-09-25T21:10:17Z 2015-09-25T21:10:17Z 10000-01-01 1999 1999 The addition of sodium acetate in MP2 medium was also able to stabilize solvent production by C. beijerinckii BA101, a hypersolvent producing mutant derived from NCIMB 8052. Moreover, BA101 demonstrated a greater increase in solvent production than NCIMB 8052 when acetate was added into MP2 medium. During the exponential growth phase, the specific activities of CoA transferase, acetoacetate decarboxylase and butyraldehyde dehydrogenase were higher in BA101 than in NCIMB 8052. mRNA expression levels of the sol operon were also elevated during the exponential and early stationary growth phases in BA101. The increased phosphotransbutyrylase activity, as a result of elevated ptb-buk operon expression, resulted in enhanced butyrate production by BA101 during the early exponential phase, which was offset by the elevated acid reassimilation due to an increase in CoA transferase activity. The results indicate that an increase in expression of the sol operon and, consequently, activities of associated enzymes may be, in part, responsible for enhanced solvent production by BA101 relative to NCIMB 8052. Made available in DSpace on 2015-09-25T21:10:17Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9952987.pdf: 6487356 bytes, checksum: 2d0663ee7472e6105dffcb6edb6ff12b (MD5) Previous issue date: 1999 Embargo set by: Seth Robbins for item 85019 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 136 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1999. http://hdl.handle.net/2142/83738 (MiAaPQ)AAI9952987 eng Biology, Microbiology Genetic and Physiological Characterization of Clostridium Beijerinckii BA101 and NCIMB 8052 text Food Science and Human Nutrition Food Science and Human Nutrition University of Illinois at Urbana-Champaign Dissertation Ph.D.