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Sligar, Stephen G. Kelley, Jennifer Gann 2015-09-25T22:13:13Z 2015-09-25T22:13:13Z 10000-01-01 2004 2004 Through an initial set of experiments high pressure SPR (HP-SPR) was used to measure changes in the reflectivity of various liquids under hydrostatic pressures ranging from ambient to 276 bar (4000 psi). The changes in refractive index as a function of increasing pressure for these solutions were compared and an indication of how these methods can be used to determine volume changes in biomolecular interactions is provided. The application of HP-SPR was extended to the use of commercially available sensor chips. We have shown that BIAcore(TM) SA chips maintain their surface binding functionality after pressure exposure and that effects to the surface are reversible to 6000 psi. Three test systems were chosen to be studied using this instrument, each having different characteristics; cytochrome c association to cytochrome b5, unfolding of staphylococcal nuclease, and binding of the BamH1 restriction enzyme to its DNA recognition site. Made available in DSpace on 2015-09-25T22:13:13Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3153349.pdf: 8082757 bytes, checksum: 2c5c603ba249e0295dc9c89a0318a026 (MD5) Previous issue date: 2004 Embargo set by: Seth Robbins for item 85434 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 163 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2004. http://hdl.handle.net/2142/84153 (MiAaPQ)AAI3153349 eng Chemistry, Analytical Development of High Pressure Surface Plasmon Resonance for the Study of Biosystems text Chemistry Chemistry University of Illinois at Urbana-Champaign Dissertation Ph.D.