2026-09-21T04:51:23Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/84233 2023-07-11 col_2142_5131 col_2142_14789 com_2142_5130 com_2142_14788 com_2142_8903

Lu, Yi Reynolds, Andrea Brown 2015-09-25T22:13:35Z 2015-09-25T22:13:35Z 10000-01-01 2006 2006 A separate in vitro selection experiment was carried out in the presence of Pb2+ in order to compare the secondary structures of the resulting DNAzymes with the structure of the 8-17-like DNAzymes, which were selected through three independent experiments in the presence of: (1) Zn2+, (2) Mg2+, and (3) histidine plus Mg2+. The DNAzymes obtained from this experiment did not appear to contain the secondary structure of the 8-17 catalytic core. The reason for this is unclear, although one possibility is that the larger random region in the initial pool may have allowed for more sequence and structural diversity in the initial pool. The DNAzymes examined displayed higher specificity and lower sensitivity for Pb2+ compared to the 8-17 DNAzyme. The sensitivity may be improved through more stringent selection conditions. This work may eventually lead to an improved Pb2+ biosensor that does not display significant activity with contaminating metal ions. Made available in DSpace on 2015-09-25T22:13:35Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3223701.pdf: 4320275 bytes, checksum: 56cc49bd6886e2e5fa4fcd3a7acc48a1 (MD5) Previous issue date: 2006 Embargo set by: Seth Robbins for item 85514 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 169 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2006. http://hdl.handle.net/2142/84233 (MiAaPQ)AAI3223701 eng Chemistry, Biochemistry In Vitro Selection and Characterization of Highly Selective Metal -Dependent DNAzymes With Potential Biosensor Applications text Chemistry Chemistry University of Illinois at Urbana-Champaign Dissertation Ph.D.