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Sweedler, Jonathan V. Scanlan, Cory Randolph 2015-09-25T22:13:39Z 2015-09-25T22:13:39Z 10000-01-01 2006 2006 A second cell-cell signaling molecule investigated is D-aspartate (D-Asp). D-Asp is present in the nervous systems of both vertebrates and invertebrates, and its biosynthesis has been observed in mammalian cells. We suspect that D-Asp acts as a classical neurotransmitter in the central nervous system. The mollusk Aplysia californica has shown high levels of D-Asp in the neural ganglia; and this, along with its relatively simple invertebrate system, makes Aplysia a viable model system for studying the function and neurochemistry of D-Asp. Using Aplysia californica , we have investigated the localization of D-Asp as well as its synthesis from L-Asp. We developed a technique utilizing off-line CE coupled with radionuclide detection, which enables high sensitivity characterization of L- to D-Asp conversion. Furthermore, these capillary electrophoretic techniques, along with liquid scintillation counting and MALDI-MS analysis, have allowed us to address other criteria required to determine whether D-Asp acts as a classical neurotransmitter, including release from cells, sodium dependence of uptake, response to D-Asp by tissues, and the D-Asp stimulated release of cardiomodulatory peptides from the R3-14 cells. These techniques developed provide robust methods for the analysis of neurotransmitters and elucidation of their chiral moieties in complex mass-limited biological samples. Made available in DSpace on 2015-09-25T22:13:39Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3250319.pdf: 6043699 bytes, checksum: 39615f1b2198a93477a8de60d168b452 (MD5) Previous issue date: 2006 Embargo set by: Seth Robbins for item 85532 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 225 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2006. http://hdl.handle.net/2142/84251 (MiAaPQ)AAI3250319 eng Chemistry, Analytical Analysis of D-Aspartate as a Signaling Molecule in the Aplysia Californica Central Nervous System Using Capillary Electrophoresis and Radioisotopic Labeling text Chemistry Chemistry University of Illinois at Urbana-Champaign Dissertation Ph.D.