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oai:www.ideals.illinois.edu:2142/84282 2023-07-11 col_2142_5131 col_2142_14789 com_2142_5130 com_2142_14788 com_2142_8903

Jonathan Sweedler Squires, Leah N. 2015-09-25T22:13:47Z 2015-09-25T22:13:47Z 10000-01-01 2007 2007 Here the metabolism of 5-HT has been investigated in higher animals, the deuterostomes, which included the invertebrates Xenoturbella bocki, a marine worm, and Strongylocentrotus purppuratus, the sea urchin as well as mammals such as Rattus norvegicus. Studies were performed using a highly specialized capillary electrophoresis (CE) system designed to detect 5-HT and related compounds at attomole levels. The invertebrate deuterstomes investigated were found to metabolize 5-HT in a manner similar to both more primitive invertebrates and to vertebrates; results include the discovery of metabolites such as gammaglutamyl 5-HT, 5-HT sulfate (5-HT-SO4) and 5-hydroxyindole acetic acid (5-HIAA) in these organisms. A novel 5-HT metabolite in the rat (Rattus norvegicus ), 5-hydroxyindole thiazolidine carboxylic acid (5-HITCA), was characterized via mass spectrometry (MS) and further investigated using CE. Levels of 5-HT and some of its key metabolites were also investigated in this mammal under conditions of induced serotonin syndrome, a physiological condition caused by an excessive increase in 5-HT levels often associated with a combination of antidepressant therapies. This research contributes to our understanding of the metabolic pathways of 5-HT in both healthy and disease states. The findings aid in understanding the 5-HT link to various disorders. Made available in DSpace on 2015-09-25T22:13:47Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3290389.pdf: 3352425 bytes, checksum: a0ebac82484a9d976ef07b9d1da614ea (MD5) Previous issue date: 2007 Embargo set by: Seth Robbins for item 85563 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 155 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2007. http://hdl.handle.net/2142/84282 (MiAaPQ)AAI3290389 eng Chemistry, Analytical Investigations of Serotonin Metabolism in Mammalian and Other Deuterostome Model Systems text Chemistry Chemistry University of Illinois at Urbana-Champaign Dissertation Ph.D.