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Kelleher, Neil L. Boyne, Michael Thomas, II 2015-09-25T22:13:53Z 2015-09-25T22:13:53Z 10000-01-01 2008 2008 And finally, a high-resolution MS/MS platform was developed to analyze large peptides at >>50,000 resolving power along with a software suite for interpreting resolved isotopic distributions and handling all known protein modifications on a high throughput basis. Called Middle Down Proteomics, this process identified 7454 peptides from 2-20 kDa (1472 unique) from 555 proteins after just 23 LC-MS/MS injections. Along with greatly increased confidence for peptide identification (expectation values from 10-89 to 104) and characterization (up to 20% of peptides were detected as modified in some LC-MS/MS runs), fragmentation data with <2 ppm accuracy also enabled error tolerant and multiplexed database searching---all clearly demonstrated in this thesis. Made available in DSpace on 2015-09-25T22:13:53Z (GMT). No. of bitstreams: 3 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3337728.pdf: 4653479 bytes, checksum: 58503ceb8cbd7c977efe575d6f0c907c (MD5) 3337728.zip: 28239659 bytes, checksum: 2329ea784cf57f7b6b431b1f6fd7e5d4 (MD5) Previous issue date: 2008 Embargo set by: Seth Robbins for item 85586 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 155 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2008. http://hdl.handle.net/2142/84305 (MiAaPQ)AAI3337728 eng Chemistry, Analytical Development and Application of Next Generation Mass Spectrometry for Protein Analysis at High-Resolution text Chemistry Chemistry University of Illinois at Urbana-Champaign Dissertation Ph.D.