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Sweedler, Jonathan V. Shippy, Scott Allen 2015-09-25T22:14:16Z 2015-09-25T22:14:16Z 10000-01-01 1997 1997 Neuropeptide release is also studied with MALDI TOF MS. In this method, a small chamber (10 $\mu$l) is used to hold an isolated bag cell cluster in a small volume of physiological saline. The chamber consists of a standard pipette tip with the narrow end melted around two sections of fused silica capillaries. Physiological saline flows into and from the chamber via capillaries and gravimetric pressure flow. Following electrical stimulation of a bag cell cluster, 50-100 nl samples are collected from the outlet end of the sampling capillary for analysis. MALDI MS is performed on the sample for peptide detection. Even in high salt concentrations peptides are detected by MALDI MS. Made available in DSpace on 2015-09-25T22:14:16Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9812771.pdf: 2707555 bytes, checksum: 1f9af55f1be80101176f1ab9d87f907e (MD5) Previous issue date: 1997 Embargo set by: Seth Robbins for item 85672 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 101 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1997. http://hdl.handle.net/2142/84391 (MiAaPQ)AAI9812771 eng Biology, Neuroscience Development of Assays for Neuronal Peptide Content and Release text Chemistry Chemistry University of Illinois at Urbana-Champaign Dissertation Ph.D.