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oai:www.ideals.illinois.edu:2142/84790 2023-07-11 col_2142_5131 col_2142_14795 com_2142_5130 com_2142_14794 com_2142_14793 com_2142_8903

Ordal, George W. Saulmon, Michael Monroe 2015-09-25T22:27:55Z 2015-09-25T22:27:55Z 10000-01-01 2002 2002 Studying the quantitative behavior of tethered B. subtilis cells led to the discovery of new functional features of several chemotaxis proteins. Results are presented that demonstrate that the functional roles of CheW and CheV are different, and that the response regulator domain of CheV plays an important role in adaptation to the addition of stimulus. Deleting the methylesterase CheB from the system had a profound effect on chemotaxis in the presence of a stimulus, as well as an effect on the duration of rotational events in the absence of stimulus. One of the most interesting discoveries was the antagonistic roles of CheC and CheD in biasing the probability of smooth swimming. CheC was identified as a regulator of the energy barrier associated with flagellar switching, and so is presumed to contact the flagellar switch. Since CheC also interacts with the receptor complex, it may migrate between the switch and receptor complexes during adaptation. Made available in DSpace on 2015-09-25T22:27:55Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3070426.pdf: 10435473 bytes, checksum: 5b02237598e00cbafba43d3a40b00f0d (MD5) Previous issue date: 2002 Embargo set by: Seth Robbins for item 86071 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 279 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2002. http://hdl.handle.net/2142/84790 (MiAaPQ)AAI3070426 eng Biology, Microbiology A Quantitative Characterization of the Chemotactic Behavior of Tethered Bacillus Subtilis Cells text Biochemistry Biochemistry University of Illinois at Urbana-Champaign Dissertation Ph.D.