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oai:www.ideals.illinois.edu:2142/84793 2023-07-11 col_2142_5131 col_2142_14795 com_2142_5130 com_2142_14794 com_2142_14793 com_2142_8903

Lies, Mark Andrew, III Gerlt, John A. 2015-09-25T22:27:56Z 2015-09-25T22:27:56Z 10000-01-01 2003 2003 Using the published operon structure of phenylacetate utilization, BLAST searches of public sequence databases, and metabolic information from a related compound, two pathways for phenylacetate metabolism were proposed. The genes encoding PaaF, PaaG, PaaH, PaaJ, and PaaI were cloned, and the proteins expressed, and purified. I showed by enzyme assays using purified proteins that beta-oxidation in phenylacetate metabolism proceeds via adipyl-CoA intermediates. I showed that PaaG catalyzes the isomerization of trans-Delta 3-hexenedioyl-CoA to trans-Delta2-hexenedioyl-CoA, uses an Asp as a general base, and the reaction proceeds without incorporation of solvent protons. Made available in DSpace on 2015-09-25T22:27:56Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3086121.pdf: 8957610 bytes, checksum: 6cc20f9b13365bbcc57c5249fe6180a3 (MD5) Previous issue date: 2003 Embargo set by: Seth Robbins for item 86074 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 199 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2003. http://hdl.handle.net/2142/84793 (MiAaPQ)AAI3086121 eng Biology, Molecular PaaG and PaaF: New Members of the Crotonase Superfamily text Biochemistry Biochemistry University of Illinois at Urbana-Champaign Dissertation Ph.D.