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oai:www.ideals.illinois.edu:2142/84794 2023-07-11 col_2142_5131 col_2142_14795 com_2142_5130 com_2142_14794 com_2142_14793 com_2142_8903

Shapiro, David J. Thulasiraman, Padmamalini 2015-09-25T22:27:57Z 2015-09-25T22:27:57Z 10000-01-01 2003 2003 In addition to pro-inflammatory agents, the synthetic anti-inflammatory glucocorticoid, dexamethasone, induced PI-9 gene expression via an imperfect glucocorticoid response element (GRE) located at -208. Dexamethasone induced PI-9 mRNA and protein. Dexamethasone-glucocorticoid receptor (GR) complex inhibited IL-1beta induced PI-9 gene expression but IL-1beta did not interfere with dexamethasone induction of PI-9. Glucocorticoid administration resulted in reduced p65 binding to a consensus NF-kappaB element. To investigate the mechanism of crosstalk between Dex-GR and NF-kappaB, we analyzed the effect of dexamethasone on induction of IkappaB-alpha, the inhibitory heterodimer of p65. Dexamethasone induced IkappaBalpha in HepG2 cells. This may be one of the mechanisms used by Dex-GR to interfere with NF-kappaB activity and block IL-1beta-induced PI-9 gene expression. Our findings show that liganded GR may regulate IL-1beta induction of PI-9 expression through NF-kappaB and AP-1 transcription factors by a mechanism quite different than that seen for estrogen-ER. Made available in DSpace on 2015-09-25T22:27:57Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3086200.pdf: 5064213 bytes, checksum: ab5cfcdf01a5cc407d42d59d4fbfb5c2 (MD5) Previous issue date: 2003 Embargo set by: Seth Robbins for item 86075 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 112 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2003. http://hdl.handle.net/2142/84794 (MiAaPQ)AAI3086200 eng Chemistry, Biochemistry Modulators of Inflammation and Steroid Receptors Regulate Proteinase Inhibitor 9 Gene Expression text Biochemistry Biochemistry University of Illinois at Urbana-Champaign Dissertation Ph.D.