2026-09-22T17:02:17Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/84814 2023-07-11 col_2142_5131 col_2142_14795 com_2142_5130 com_2142_14794 com_2142_14793 com_2142_8903

William W. Mantulin Cheng, Melanie Anne B. 2015-09-25T22:28:02Z 2015-09-25T22:28:02Z 10000-01-01 2005 2005 Fluctuation correlation spectroscopy (FCS) measurements performed on the intact BBM reveal that the protein-lipid dynamics in renal BBM is complex with a large range of diffusing species. In particular, the dynamics of NaPi-IIa was related to the perturbations in the BBM as a result of dietary potassium deficiency. By calculating the diffusion coefficient of NaPi-IIa and using photon counting histogram (PCH) analysis to measure protein aggregation, we determined that the lateral diffusion of NaPi-IIa is slowed down and its aggregation is increased in potassium deficiency, both of which are associated with the decreased Na/Pi cotransport activity. This model was proposed as a means for posttranslational regulation of Na/Pi cotransport activity that is mediated by the increased sphingomyelin and glycosphingolipid content in potassium-deficient BBM, although the mechanisms by which these lipids may modulate NaPi-IIa activity have yet to be determined. Made available in DSpace on 2015-09-25T22:28:02Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3198946.pdf: 3481239 bytes, checksum: 294e1ceea9ec48acfc8cddce47ac10c1 (MD5) Previous issue date: 2005 Embargo set by: Seth Robbins for item 86095 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 126 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005. http://hdl.handle.net/2142/84814 (MiAaPQ)AAI3198946 eng Biology, Cell Characterization of Membrane Organization and Dynamics in Giant Unilamellar Vesicles Assembled From Rat Kidney Membrane Extracts and Fragments text Biochemistry Biochemistry University of Illinois at Urbana-Champaign Dissertation Ph.D.