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Gerlt, John A. Millikin, Cheri Lynn 2015-09-25T22:28:03Z 2015-09-25T22:28:03Z 10000-01-01 2005 2005 The dehydration of both D-mannonate, via syn-elimination, and D-altronate, via anti-elimination, is analogous to the stereochemical promiscuity engineered into the GlucD active site. The redesign of GlucD and the characterization of RspA illustrate the same type of promiscuity: syn and anti dehydrations in the same active site with different substrates, which results from proton abstraction of stereochemically equivalent protons. These experiments illustrate how Nature could have utilized the (beta/alpha)8-barrel as a scaffold to evolve new activities. Made available in DSpace on 2015-09-25T22:28:03Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3199089.pdf: 7424041 bytes, checksum: 5739157f992a09e43550062f7ac12817 (MD5) Previous issue date: 2005 Embargo set by: Seth Robbins for item 86099 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 256 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005. http://hdl.handle.net/2142/84818 (MiAaPQ)AAI3199089 eng Chemistry, Biochemistry Natural and Engineered Promiscuity Within the Enolase Superfamily text Biochemistry Biochemistry University of Illinois at Urbana-Champaign Dissertation Ph.D.