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oai:www.ideals.illinois.edu:2142/84819 2023-07-11 col_2142_5131 col_2142_14795 com_2142_5130 com_2142_14794 com_2142_14793 com_2142_8903

Huang, Raven H. Xie, Wei 2015-09-25T22:28:03Z 2015-09-25T22:28:03Z 10000-01-01 2005 2005 Part II. Hypermodification of base adenosine at position 37. Hypermodification of A37 (position 3' adjacent to the anticodon region) starts with the formation of (isopentenyl)-adenosine (i6A) and in E. coli, the first step is catalyzed by the enzyme MiaA. Through BLAST search, a few protein sequences highly homologous to MiaA were identified. These protein-encoding genes were cloned, over-expressed and purified. Enzymatic assays were carried out to test the putative enzymes but no activities were detected. The failure to detect activities might result from incorrect assay conditions or substrates and more data is yet to be acquired on this aspect. Crystals of the P. aeruginosa protein were obtained and its structure was determined at 2.2 A. Although the electron density of a segment of the protein was not observed, the partial structure nevertheless shows a central channel composed of positive charged residues. A hypothesis of the MiaA catalytic mechanism is proposed. Made available in DSpace on 2015-09-25T22:28:03Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3199180.pdf: 3370435 bytes, checksum: 6f5b40618e82c17a007d385bbb56225e (MD5) Previous issue date: 2005 Embargo set by: Seth Robbins for item 86100 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 116 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005. http://hdl.handle.net/2142/84819 (MiAaPQ)AAI3199180 eng Chemistry, Biochemistry Biochemical and Structural Studies of TGT and MiaA: Key Enzymes Involved in Two Types of Hypermodifications text Biochemistry Biochemistry University of Illinois at Urbana-Champaign Dissertation Ph.D.