2026-09-21T06:30:42Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/84871 2023-07-11 col_2142_5131 col_2142_14795 com_2142_5130 com_2142_14794 com_2142_14793 com_2142_8903

2010 Hergenrother, Paul J. Heeres, James T. 2015-09-25T22:28:16Z 2015-09-25T22:28:16Z 10000-01-01 2010 "High-throughput screening (HTS) has played an integral role in the discovery of lead molecules that eventually are transformed into the therapies of tomorrow. Contributing towards the success of any screening campaign is the target choice, chemical library size and diversity, as well as the technology utilized in screening. Photonic crystal (PC) biosensors are a relatively new technology whose potential is only beginning to be realized. PC biosensors are a ""label-free"" technology compatible with standard high-throughput techniques. Macromolecular interactions including protein---small molecule, protein---nucleic acid, protein---protein, and whole cell interactions can be measured using PC biosensors. Presented herein is the first application of PC biosensors to a protein---nucleic acid interaction and a simplified protein---protein interaction platform. The protein---nucleic acid interaction targeted is the apoptosis inducing factor (AIF)---DNA interaction, which is critical for the mediation of caspaseindependent cell death in neurodegenerative disease and stroke. Protein---protein interactions were targeted by modifying the PC biosensor surface for detection of the His6 tag, which is widely employed in protein purification." Made available in DSpace on 2015-09-25T22:28:16Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3452172.pdf: 36241002 bytes, checksum: 217c3bd0954addb5bfdedf0df6966b9c (MD5) Previous issue date: 2010 Embargo set by: Seth Robbins for item 86152 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 242 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2010. http://hdl.handle.net/2142/84871 (MiAaPQ)AAI3452172 eng Chemistry, Biochemistry High-Throughput Detection of Protein---Protein and Protein---Dna Interactions via Photonic Crystal Biosensors: Applications to Apoptosis Inducing Factor text Biochemistry Biochemistry University of Illinois at Urbana-Champaign Dissertation Ph.D.