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oai:www.ideals.illinois.edu:2142/84875 2023-07-11 col_2142_5131 col_2142_14795 com_2142_5130 com_2142_14794 com_2142_14793 com_2142_8903

Kranz, David M. Brodnicki, Thomas Charles 2015-09-25T22:28:17Z 2015-09-25T22:28:17Z 10000-01-01 1997 1997 Mutant 2C scTCRs were further examined in ligand binding experiments. The 2C TCR recognizes a peptide (QL9) presented by the MHC product L$\sp{\rm d}$. Four alanine mutations which affected mAb binding also disrupted binding to the QL9/L$\sp{\rm d}$ complex by greater than ten-fold relative to the wild-type scTCR. A fifth $\alpha$ chain mutation, located within a loop that is analogous to the fourth hypervariable region (HV4) of the $\beta$ chain, disrupted binding to QL9/L$\sp{\rm d}$ approximately five-fold. Based on these results and a homology model of the 2C $\alpha$ chain variable region, these five residues are likely to be involved in either stabilization of CDR loops or in direct contact with the peptide/MHC ligand. Made available in DSpace on 2015-09-25T22:28:17Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9717257.pdf: 8485594 bytes, checksum: 0144c71bd1505211d340bcfd5f8d4cdb (MD5) Previous issue date: 1997 Embargo set by: Seth Robbins for item 86156 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 195 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1997. http://hdl.handle.net/2142/84875 (MiAaPQ)AAI9717257 eng Health Sciences, Immunology T Cell Receptor Alpha Chain Residues Involved in Antibody Binding and Recognition of peptide/MHC Ligand text Biochemistry Biochemistry University of Illinois at Urbana-Champaign Dissertation Ph.D.