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Peter A.B.Orlean Costello, Lisa Catherine 2015-09-25T22:28:24Z 2015-09-25T22:28:24Z 10000-01-01 1998 1998 Next, it was desired to obtain structural information on in vivo GPI anchor intermediates. To address this issue, I exploited the fact that at non-permissive temperature, the yeast dpm1--6 mutant accumulates GlcNH2-(acyl-inositol)-Pl. This precursor molecule is the earliest biosynthetic intermediate that can be detected in in vivo radiolabeling experiments. I isolated this lipid and had it analyzed by liquid chromatography - mass spectroscopy (LC-MS) with an electrospray interface. The results show that this intermediate has very long chain fatty acids, most likely on its diacylglycerol moiety. This has significant implications for GPI anchor biosynthesis in yeast and suggests that GPI anchors are either assembled on a specialized PI or that the diacylglycerol moiety on early GPI precursor molecules are rapidly remodeled. Made available in DSpace on 2015-09-25T22:28:24Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9912214.pdf: 4823771 bytes, checksum: ec86be2c1acdde5d76fc3245bb7b38d0 (MD5) Previous issue date: 1998 Embargo set by: Seth Robbins for item 86177 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 125 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1998. http://hdl.handle.net/2142/84896 (MiAaPQ)AAI9912214 eng Biology, Molecular Biosynthesis and Characterization of Early Glycosylphosphatidylinositol Anchor Intermediates in Saccharomyces Cerevisiae text Biochemistry Biochemistry University of Illinois at Urbana-Champaign Dissertation Ph.D.