2026-09-25T07:00:35Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/85049 2023-07-11 col_2142_5131 col_2142_14798 com_2142_5130 com_2142_686 com_2142_397

Shaw, Paul D. Sotak, Robert 2015-09-25T22:33:37Z 2015-09-25T22:33:37Z 10000-01-01 1997 1997 In planta growth curves showed that PTBR4.000 could not reach the same population level in tobacco or green bean leaves as PTBR4.000. A 4.2 kb Hin dIII-Eco RI fragment cloned downstream of a vector promoter was able to restore pathogenicity to PTBR4.000 in intact tobacco leaves. ORF1 and ORF2 were found not to be required for pathogenicity of tobacco since a marker exchange mutation in these ORFs remained pathogenic. The combined data indicate that ORF3 probably was mutated in a polar manner by Tn5 resulting in the loss of tobacco pathogenicity in PTBR4.000. Based on the similarity of ORF3 to an aspartate aminotransferase and the observed phenotypic differences it was proposed that PTBR4.000 is defective in nitrogen metabolism. Made available in DSpace on 2015-09-25T22:33:37Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9737259.pdf: 5861306 bytes, checksum: 039d8a21428c528914cbe01748d9e203 (MD5) Previous issue date: 1997 Embargo set by: Seth Robbins for item 86330 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 177 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1997. http://hdl.handle.net/2142/85049 (MiAaPQ)AAI9737259 eng Biology, Molecular Characterization of a Pathogenicity Gene in Pseudomonas Syringae Pv. Tabaci Strain PTBR2.024 text Crop Sciences Crop Sciences University of Illinois at Urbana-Champaign Dissertation Ph.D.