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oai:www.ideals.illinois.edu:2142/85052 2023-07-11 col_2142_5131 col_2142_14798 com_2142_5130 com_2142_686 com_2142_397

Widholm, Jack M. Chanprame, Sermsiri 2015-09-25T22:33:38Z 2015-09-25T22:33:38Z 10000-01-01 1998 1998 To modify soybean sugar content, somatic embryos of soybean cvs. Peterson Jade, Daelip Hakja (PI 157.419), Yakumo Meaka (PI 417.468), Kinshu (PI 549.065) Kaohsiung No.1 (PI 536.546) and Jack were transformed with yeast invertase (SUC 2) and $\alpha$-galactosidase (agl A) from Aspergillus niger under the control of the lectin promoter using particle bombardment. The transformation was not very successful. In some cultivars, no green clumps were obtained after 8 weeks of selection in FG medium containing 25 $\mu$g/ml hygromycin B. The green clumps obtained from transformation and selection were subjected to PCR analysis for the transgenes. Some lines were PCR positive for the hygromycin resistance and/or $\alpha$-galactosidase genes but Southern and northern analysis gave negative results. These results suggest that the transformed genes were lost during the somatic embryo multiplication due to the friability of some green clumps that were discarded during the culture process. Made available in DSpace on 2015-09-25T22:33:38Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9834660.pdf: 4231123 bytes, checksum: 6ac2de604853ef9b1b6871d89ba86c92 (MD5) Previous issue date: 1998 Embargo set by: Seth Robbins for item 86333 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 97 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1998. http://hdl.handle.net/2142/85052 (MiAaPQ)AAI9834660 eng Agriculture, Plant Culture Modification of Vegetable Soybean Sugar Content via Gene Transformation text Crop Sciences Crop Sciences University of Illinois at Urbana-Champaign Dissertation Ph.D.