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Beasley, Val Richard Hall, Jeffery Owen 2015-09-25T22:43:02Z 2015-09-25T22:43:02Z 10000-01-01 1997 1997 Cellular effects of monensin, laidlomycin, and LP were evaluated after 24-hour exposure in 3-day (myoblasts) and 14-day ($>$90% myotubes) cultures of E-63 rat skeletal muscle cells. Each of these ionophores induced dose-dependent increases in lactate concentrations, decreases in pyruvate concentrations, and decreases in accumulations of NR. Cultures of both myoblasts and myotubes responded similarly, except that myoblasts had greater lactate concentrations than myotubes. Minimum toxic concentrations were 0.1 $\mu$M for each ionophore in both ages of cultures. Concentrations that inhibited NR accumulation by 50% were similar for all three ionophores in both ages of cultures. However, cytotoxic effects of these ionophores were at least partially reversible, as indicated by significant increases in NR accumulation after ionophore removal and incubation for an additional 24 hours. Cytotoxicity of monensin or LP, as indicated by NR accumulation and lactate concentrations, was not affected by decreased media concentrations of potassium, sodium, or calcium. Similarly, cytotoxicity was not influenced by addition of nifedipine, verapamil, or magnesium. Made available in DSpace on 2015-09-25T22:43:02Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9717281.pdf: 16995561 bytes, checksum: 88ff9b75452c1c71e7821524b4a60b51 (MD5) Previous issue date: 1997 Embargo set by: Seth Robbins for item 86669 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 317 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1997. http://hdl.handle.net/2142/85388 (MiAaPQ)AAI9717281 eng Biology, Veterinary Science Mechanisms of Ionophore Toxicoses: Laidlomycin Propionate Toxicosis in the Horse and Monensin, Laidlomycin, and Laidlomycin Propionate in Muscle Cultures text Biology Biology University of Illinois at Urbana-Champaign Dissertation Ph.D.