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2015-09-25T22:43:03Z 10000-01-01 1997 1997 Horwitz, Alan F. McDonald, Kevin Alan 2015-09-25T22:43:03Z We have also isolated a monoclonal antibody, P4B2, which localizes to multiple adhesion junctions, namely, a subset of focal adhesions, the Z-disc of muscle, and neuromuscular junctions. Immunopurification of the antigen to this antibody from chicken brain tissue yielded a complex of three prominent proteins with mobilities of 36, 30 and 18 kD. Amino acid sequencing of the purified proteins identified the 36 kD protein as GAPDH. The other 2 protein bands were heterogeneous, containing proteins found in the synaptic vesicle fusion core complex. Immunolocalization of P4B2 antigen in developing cultured muscle cells showed that the antigen is incorporated into Z-lines soon after the sarcomeric architecture was positive for $\alpha$-actinin. Together, the data indicate the P4B2 antigen is part of a unique GAPDH containing protein complex that may be involved in reinforcement of established cytoskeletal structures. Made available in DSpace on 2015-09-25T22:43:03Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9737192.pdf: 6681135 bytes, checksum: e1e9c435e2977b03e5a1c88af37cc4c2 (MD5) Previous issue date: 1997 Embargo set by: Seth Robbins for item 86672 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 158 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1997. http://hdl.handle.net/2142/85391 (MiAaPQ)AAI9737192 eng Biology, Molecular Junction Specificity and Transmembrane Linkages of Integrins text Biology Biology University of Illinois at Urbana-Champaign Dissertation Ph.D.