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oai:www.ideals.illinois.edu:2142/85466 2023-07-11 col_2142_5131 col_2142_14828 com_2142_5130 com_2142_14827 com_2142_14793 com_2142_8903

2015-09-25T22:46:11Z 2015-09-25T22:46:11Z 10000-01-01 2007 2007 Selvin, Paul R. Toprak, Erdal Finally, we used a single molecule fluorescence quenching assay to study the timing of the neck linker conformational change that occurs as kinesin walks processively along a microtubule. We observed repetitive, alternating cycles of fluorescence and quenching as individual kinesin motors walked along microtubules. By monitoring the position of the kinesin during these fluorescence cycles, we were able to conclude that one cycle of quenching/unquenching occurs with each 8 nm step, and that each step corresponds to an ATP-induced separation of neck linkers. Made available in DSpace on 2015-09-25T22:46:11Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3301237.pdf: 3002924 bytes, checksum: f81555e57842d9493b91f2073181ae8a (MD5) Previous issue date: 2007 Embargo set by: Seth Robbins for item 86747 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 135 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2007. http://hdl.handle.net/2142/85466 (MiAaPQ)AAI3301237 eng Biophysics, General Fluorescent Tools for Watching Conformational Changes of Biological Molecules text Biophysics and Computational Biology Biophysics and Computational Biology University of Illinois at Urbana-Champaign Dissertation Ph.D.