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oai:www.ideals.illinois.edu:2142/85476 2023-07-11 col_2142_5131 col_2142_14828 com_2142_5130 com_2142_14827 com_2142_14793 com_2142_8903

Martin Gruebele Liu, Feng 2015-09-25T22:46:13Z 2015-09-25T22:46:13Z 10000-01-01 2009 2009 Downhill protein folding, i.e., folding with the free energy barrier comparable to the thermal energy kBT, is investigated by combining laser induced temperature-jump kinetics measurements and Langevin dynamics simulations. A kinetic and thermodynamic survey of multiple WW domain mutants is performed in combination with a model to reveal the correlation between protein stability and downhill folding propensity. A designed histidine containing lambda repressor mutant is identified to be a downhill folder even at its melting temperature, showing both thermodynamic and kinetic signatures of downhill folding for the first time. All the reported downhill folders are classified based on their typical downhill folding free energy building blocks using Langevin dynamics simulations. Made available in DSpace on 2015-09-25T22:46:13Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3363020.pdf: 2428191 bytes, checksum: ab97d71302d297b460f89d9aa0a2033f (MD5) Previous issue date: 2009 Embargo set by: Seth Robbins for item 86757 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 140 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2009. http://hdl.handle.net/2142/85476 (MiAaPQ)AAI3363020 eng Biophysics, General Exploring Downhill Protein Folding Free Energy Landscapes text Biophysics and Computational Biology Biophysics and Computational Biology University of Illinois at Urbana-Champaign Dissertation Ph.D.