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Gratton, Enrico Chen, Yan 2015-09-25T22:46:14Z 2015-09-25T22:46:14Z 10000-01-01 1999 1999 Binary dye mixtures were used to demonstrate the influence of the molecular brightness on the fluctuation amplitude G(0). This dependence allows to resolve multiple species based on the difference in their molecular brightness, which we applied to a titration study of an antibody with its ligand. The global fit of the average photon counts ⟨k⟩ and the fluctuation amplitude G(0) as a function of the protein concentration determined the number of binding sites and the dissociation coefficient KD. The analysis further demonstrated heterogeneity of the antibody complex on a time scale not easily accessible by other techniques. Made available in DSpace on 2015-09-25T22:46:14Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9944818.pdf: 3181075 bytes, checksum: faf8b373ea19df4ddc246d86aa3eddf7 (MD5) Previous issue date: 1999 Embargo set by: Seth Robbins for item 86769 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 136 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1999. http://hdl.handle.net/2142/85488 (MiAaPQ)AAI9944818 eng Statistics Analysis and Applications of Fluorescence Fluctuation Spectroscopy text Biophysics and Computational Biology Biophysics and Computational Biology University of Illinois at Urbana-Champaign Dissertation Ph.D.