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oai:www.ideals.illinois.edu:2142/86305 2023-07-11 col_2142_5131 col_2142_14855 com_2142_5130 com_2142_14854 com_2142_14793 com_2142_8903

Chiba, Akira Siechen, Scott A. 2015-09-28T15:03:16Z 2015-09-28T15:03:16Z 10000-01-01 2006 2006 "Individual neurons extend multiple processes whose growth cones exhibit different responses to their environment. In culture, detached growth cones display guidance autonomy, contain mRNA for cytoskeletal and other axonal components, and are capable of synthesizing protein locally. However, the extent to which growth cone's autonomy contributes to its pathfinding function within the complex in vivo environment is unknown. Here, we show that detached axonal growth cones from identified Drosophila motoneurons maintain balanced filopodial activities as they extend, navigate and target postsynaptic partner cells normally. After detachment, the growth cones continue to synthesize the synaptic vesicle protein Synaptotagmin but, upon contacting targets, fail to concentrate it at the presynaptic site normally. However, if held by a micropipette that resupplies mechanical tension, the growth cones' ability to localize synaptic vesicles is restored. Our results demonstrate functional autonomy of axonal growth cones as they navigate and initiate synaptogenesis, while implicating intercellular tension as a novel ""retrograde"" mechanism to adjust synaptic function." Made available in DSpace on 2015-09-28T15:03:16Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3223717.pdf: 1032107 bytes, checksum: 65d33bbbfbe2e51b2210c3c126ff98fd (MD5) Previous issue date: 2006 Embargo set by: Seth Robbins for item 87586 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 45 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2006. http://hdl.handle.net/2142/86305 (MiAaPQ)AAI3223717 eng Biology, Cell Axon Guidance Autonomy and Tensional Requirement for Synaptic Function: Is Learning a Forced Response text Cell and Developmental Biology Cell and Developmental Biology University of Illinois at Urbana-Champaign Dissertation Ph.D.