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oai:www.ideals.illinois.edu:2142/86311 2023-07-11 col_2142_5131 col_2142_14855 com_2142_5130 com_2142_14854 com_2142_14793 com_2142_8903

Cameron, Jo Ann Andres, Robert Douglas 2015-09-28T15:03:18Z 2015-09-28T15:03:18Z 10000-01-01 2008 2008 In the current study, we examined whether treatment with Ang II regulates NMDA-mediated current, signaling and apoptosis. Western blot analysis revealed that Ang II, acting primarily through AT2 receptor activation of the protein phosphatase 2A (PP2A), attenuated NMDA-mediated increases in extracellular regulated kinase-2 (ERK-2) activity. We have identified that AT2 receptor activation inhibits cleavage of the DNA repair enzyme poly-(ADP-ribose) polymerase (PARP) following treatment with NMDA. Inhibition of ERK with MEK inhibitors (PD98059 and U0126) significantly decreased PARP cleavage (cPARP), while inhibition of PP2A with 10 nM okadaic acid, significantly increased NMDA-mediated cPARP expression. Further, we demonstrate that the AT2 receptor attenuates NMDA-mediated subunit phosphorylation and current to decrease intracellular Ca2+ levels and subsequent downstream signaling. Taken together these results characterize a novel mechanism for describing the AT2 receptor's neuroprotective functions. Made available in DSpace on 2015-09-28T15:03:18Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3314723.pdf: 1367339 bytes, checksum: 25bf0f9a3d9becf84481ee473502444f (MD5) Previous issue date: 2008 Embargo set by: Seth Robbins for item 87592 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 85 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2008. http://hdl.handle.net/2142/86311 (MiAaPQ)AAI3314723 eng Biology, Cell Angiotensin Type-2 (At2) Receptor Modulation of Nmda-Mediated Current, Signaling and Apoptosis text Cell and Developmental Biology Cell and Developmental Biology University of Illinois at Urbana-Champaign Dissertation Ph.D.