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oai:www.ideals.illinois.edu:2142/86382 2023-07-11 col_2142_5131 col_2142_16278 com_2142_5130 com_2142_16277 com_2142_9614

2006 2006 10000-01-01 Jeff Woods Lowder, Thomas W. 2015-09-28T15:04:24Z 2015-09-28T15:04:24Z We have previously shown that moderate exercise for 4 days during a mounting immune response significantly increases survival during influenza virus (A/PR/8/34) infection in mice. We hypothesized that this brief duration of exercise resulted in a decreased level of lung histopathology while altering the immune response from a Th1 (inflammatory) to a Th2 (Anti-inflammatory) response. Adult male Balb/cByJ mice (5-6mo) were inflected with 50uL of A/PR/8/34 influenza virus (40HAU) intranasally under light anesthesia (Isoflurane) and randomized to either an exercise (EX) or sedentary (SED) group. EX mice performed 20-3min of moderate exercise (8-12m/min) on a motorized treadmill 4hr post-infection and then exercised similarly for 4 consecutive days. SED mice were exposed to similar environmental conditions but did not exercise. Mice from both EX and SED groups were sacrificed 1, 3, or 5 days post-infection lungs, mediastinal lymph nodes and spleens were harvested. We found a profound reduction in IFN-gamma in lungs of EX mice when compared to SED mice 3 and 5 days post-infection. There was also a reduction in viral gene mRNA expression, although not at the level of statistical significance (p=0.241). While there were no statistically significant differences in percentage or absolute number of CED8+ cells in the lungs of infected mice, EX mice had significantly fewer cells compared with SED mice 5 days post-infection. Interestingly, we found no observable difference in lung histopathology between groups. These data suggest that moderate exercise shifts the immune response from a Th1 to a Th2 profile in mice infected with influenza virus. This exercise-induced shift in immune response may be responsible for improved survival after influenza virus infection. Made available in DSpace on 2015-09-28T15:04:24Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3250283.pdf: 7693744 bytes, checksum: 0272c41becc9d6e401511a88ad032f28 (MD5) Previous issue date: 2006 Embargo set by: Seth Robbins for item 87663 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 234 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2006. http://hdl.handle.net/2142/86382 (MiAaPQ)AAI3250283 eng Biology, Physiology Effects of Exercise on Immune Function in Young, Adult, and Aged Mice: Increased Survival and a Decrease in Inflammation text Kinesiology and Community Health Kinesiology and Community Health University of Illinois at Urbana-Champaign Dissertation Ph.D.