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oai:www.ideals.illinois.edu:2142/86630 2023-07-11 col_2142_5131 col_2142_16338 com_2142_5130 com_2142_16337 com_2142_14793 com_2142_8903

Stanley Maloy Gomez-Curet, Ilsa 2015-09-28T15:17:02Z 2015-09-28T15:17:02Z 10000-01-01 2001 2001 A modification of the S&barbelow;ystematic E&barbelow;volution of L&barbelow;igands by Exponential Enrichment (SELEX) approach was used to enrich for put control DNA molecules defective in PutA binding in vitro. These experiments identified nucleotide residues that may be important for the specificity of the PutA-put control region interaction. Using the lambda Red system, the wild-type put control region in the LT2 chromosome was replaced with the mutant put control region isolated by SELEX. The effects of the mutations in vivo was analyzed by proline dehydrogenase assays and beta-galactosidase assays using a putA::MudJ fusion downstream of each of the put control region mutant. Although the mutations isolated in the put control region are defective in PutA binding in vitro, they do not show a strong defect in vivo.. Made available in DSpace on 2015-09-28T15:17:02Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3023064.pdf: 10350223 bytes, checksum: 8afd98719c9977e562363c12b4ef4b62 (MD5) Previous issue date: 2001 Embargo set by: Seth Robbins for item 87911 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 245 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2001. http://hdl.handle.net/2142/86630 (MiAaPQ)AAI3023064 eng Biology, Molecular Protein -Dna Interactions Required for Regulation of the Proline Utilization Operon in Salmonella text Microbiology Microbiology University of Illinois at Urbana-Champaign Dissertation Ph.D.