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Imlay, James A. Woodmansee, Anh Nguyen 2015-09-28T15:17:09Z 2015-09-28T15:17:09Z 10000-01-01 2002 2002 The presence of nitric oxide (NO) greatly accelerates the rate at which hydrogen peroxide (H2O2) kills E. coli. The goal of this study was to determine the mechanism of this synergism. The filamentation of the dead cells, and their protection by cell-permeable iron chelators, indicated that NO/H2O2 killed cells by damaging their DNA through the Fenton reaction. NO also blocked respiration, an event which previous studies have shown can stimulate oxidative DNA damage. The resultant accumulation of NADH accelerates the reduction of free flavins by flavin reductase, and these reduced flavins drive Fenton chemistry by tranferring electrons to free iron. The possibility that H2O2 and NO synergize when macrophages attack captive bacteria is discussed. Made available in DSpace on 2015-09-28T15:17:09Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3070482.pdf: 5717344 bytes, checksum: 2b1d4d9095327e76688ba5b3b43fa57c (MD5) Previous issue date: 2002 Embargo set by: Seth Robbins for item 87927 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 122 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2002. http://hdl.handle.net/2142/86646 (MiAaPQ)AAI3070482 eng Biology, Microbiology The Mechanism of Oxygen and Nitric Oxide Toxicity in Escherichia Coli text Microbiology Microbiology University of Illinois at Urbana-Champaign Dissertation Ph.D.