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Abigail Salyers Gupta, Anamika 2015-09-28T15:17:19Z 2015-09-28T15:17:19Z 10000-01-01 2004 2004 CTnDOT is a 65 kb Bacteroides CTn that carries genes encoding for tetracycline resistance and erythromycin resistance. Previous studies had shown that the attDOT recombination site and integrase of CTnDOT (IntDOT) are sufficient and essential for integration in vivo. Even though the integrase of CTnDOT belongs to the lambda integrase family of recombinases, the mechanism of integration of CTnDOT is different from that of phage lambda, the model for site-specific recombination systems. Although IntDOT is sufficient for integration in vivo, recent studies have shown that a host factor is required for efficient integration. Using the gel shift assay technique, we have shown that IntDOT binds attDOT weakly and binding of IntDOT is enhanced in the presence of IHF. We propose that IHF bends attDOT DNA during recombination, facilitating the binding of CTnDOT integrase to attDOT. Using a combination of the in vivo integration assay and the gel shift assay, attDOT has been narrowed to 266 bp. We have identified the sites where IntDOT and IHF bind to attDOT using the footprinting technique. Made available in DSpace on 2015-09-28T15:17:19Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3153309.pdf: 10439938 bytes, checksum: 05625c4771821ab8e3ecc2e0e8b61f98 (MD5) Previous issue date: 2004 Embargo set by: Seth Robbins for item 87951 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 133 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2004. http://hdl.handle.net/2142/86670 (MiAaPQ)AAI3153309 eng Biology, Molecular Bacteroides Conjugative Transposons: Ecology and Mechanisms text Microbiology Microbiology University of Illinois at Urbana-Champaign Dissertation Ph.D.