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oai:www.ideals.illinois.edu:2142/86688 2023-07-11 col_2142_5131 col_2142_16338 com_2142_5130 com_2142_16337 com_2142_14793 com_2142_8903

Salyers, Abigail A. Moon, Kyung 2015-09-28T15:17:26Z 2015-09-28T15:17:26Z 10000-01-01 2006 2006 In order to examine which genes are responsible for excision, I first demonstrated that the excision genes orf2c, orf2d, orf3, and exc are part of an operon (the orf2c operon) and that this operon is regulated at the transcriptional level. I found that RteC is an DNA-binding activator of the orf2c operon and that RteA and RteB participate in excision by stimulating transcription of rteC. In order to determine whether CTnDOT regulatory genes can affect the expression of chromosomal genes in B. thetaiotaomicron , I used GeneChip and quantitative RT-PCR analyses. I showed that RteA and RteB are global regulators that induce the expression of various genes, in particular, tra genes in CTn, a cryptic CTn found in the chromosome. In addition, I characterized the repressor region, rteR, which regulates the expression of tra genes, using site-directed mutagenesis and Northern blot analysis. This region turned out to contain a small non-coding regulatory RNA that has been renamed as RteR. However, further work has to be done to examine its mechanism of action. Made available in DSpace on 2015-09-28T15:17:26Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3242945.pdf: 4902563 bytes, checksum: 11dfbeb8942266ed25216ecac6c93499 (MD5) Previous issue date: 2006 Embargo set by: Seth Robbins for item 87969 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 197 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2006. http://hdl.handle.net/2142/86688 (MiAaPQ)AAI3242945 eng Biology, Molecular Regulatory Genes of the Bacteroides Conjugative Transposon, CTnDOT text Microbiology Microbiology University of Illinois at Urbana-Champaign Dissertation Ph.D.