2026-09-21T20:30:15Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/86689 2023-07-11 col_2142_5131 col_2142_16338 com_2142_5130 com_2142_16337 com_2142_14793 com_2142_8903

Salyers, Abigail A. Romero-Guss, Melanie Claire 2015-09-28T15:17:27Z 2015-09-28T15:17:27Z 10000-01-01 2006 2006 I implemented a biochemical approach utilizing heparin column, size-exclusion and DNA-affinity chromatography, 2-D gel electrophoresis and mass fingerprinting to identify proteins that bound the attachment sites of CTnDOT (attDOT ) and NBU1 (attNI), and stimulated integration in vitro for both DNA elements. The proteins isolated and characterized include BHP, a non-specific DNA-binding protein that is most likely involved in nucleoid organization, BHP2, a homolog to BHP that probably functions in a similar capacity, and BT 1847, a specific DNA-binding protein that is a member of the AraC/Xy1S transcription regulator family. Preliminary data suggest that another protein, BT1271, works in conjunction with BT1847 to confer DNA-binding and specificity. BT1271 is annotated in the genome as the 30S ribosomal subunit S16. Made available in DSpace on 2015-09-28T15:17:27Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3242974.pdf: 3402956 bytes, checksum: 80d410a2441a15f7d30a3429329efce2 (MD5) Previous issue date: 2006 Embargo set by: Seth Robbins for item 87970 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 127 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2006. http://hdl.handle.net/2142/86689 (MiAaPQ)AAI3242974 eng Biology, Microbiology DNA -Binding Proteins That Facilitate Integration of Transmissible DNA Elements in Bacteroides Thetaiotaomicron text Ph.D. Microbiology Microbiology University of Illinois at Urbana-Champaign Dissertation