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Opperman, Kay Keyer 2015-09-28T15:17:38Z 2015-09-28T15:17:38Z Imlay, James A. 10000-01-01 1997 1997 Peroxynitrite (ONOO$\sp{-}),$ a toxic biomolecule produced from the reaction of superoxide and nitric oxide (NO$\cdot)$ in the phagolysosome, may also be toxic by the same mechanism as O$\sb2{-}.$ Our in vivo results indicated that peroxynitrite has the same intracellular targets as superoxide. Of nineteen enzymes tested, only the dehydratase enzymes containing these iron-sulfur clusters were significantly affected by peroxynitrite challenge. These iron-sulfur clusters could be repaired, and both iron storage proteins and iron import seem to be necessary for efficient repair. This enzyme inactivation led to an increased intracellular free iron. However, unlike superoxide. the free iron was rapidly sequestered. Made available in DSpace on 2015-09-28T15:17:38Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9812724.pdf: 6138161 bytes, checksum: fd59b0b7b2074ec87993ed754678008d (MD5) Previous issue date: 1997 Embargo set by: Seth Robbins for item 88005 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 156 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1997. http://hdl.handle.net/2142/86724 (MiAaPQ)AAI9812724 eng Chemistry, Biochemistry The Roles of Peroxynitrite and Superoxide in Oxidative Damage to E. Coli text Microbiology Microbiology University of Illinois at Urbana-Champaign Dissertation Ph.D.