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oai:www.ideals.illinois.edu:2142/86747 2023-07-11 col_2142_5131 col_2142_16338 com_2142_5130 com_2142_16337 com_2142_14793 com_2142_8903

Slauch, James M. Mann, Brandon Alexander 2015-09-28T15:17:48Z 2015-09-28T15:17:48Z 10000-01-01 1999 1999 To study Salmonella pathogenesis we used the IVET ( in vivo expression technology) system to select S. typhimurium genes that are specifically induced in the host during infection. This unique IVET selection was done in hopes of finding genes specifically induced in the aorta of BALB/c mice. In this selection, several genes important for the infection process were identified, as well as several other interesting genes that have no known function. One of which is defined as Salmonella -specific. The reason for using the aorta is based on the fact that in humans Salmonella species can infect healthy aortic endothelial tissue causing possible aneurysm formation. We can culture S. typhimurium from aortas of mice after an intracardiac inoculation. Histologic examination of infected tissues suggests that initially the infection starts in the periaortic adipose tissue and then proceeds towards the muscle layer. We can also directly visualize the presence of S. typhimurium in the aorta by using an indirect immunohistochemical staining procedure which shows a direct correlation between the presence of bacteria and neutrophils in the infected tissue. Made available in DSpace on 2015-09-28T15:17:48Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9944933.pdf: 5886693 bytes, checksum: 67dae09ee54ab817c2a2930a2c3d6b98 (MD5) Previous issue date: 1999 Embargo set by: Seth Robbins for item 88028 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 145 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1999. http://hdl.handle.net/2142/86747 (MiAaPQ)AAI9944933 eng Health Sciences, Pathology A Multifaceted Approach to Study Salmonella Typhimurium Pathogenesis text Microbiology Microbiology University of Illinois at Urbana-Champaign Dissertation Ph.D.