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Chiba, Akira Wolf, Brian David 2015-09-28T15:17:55Z 2015-09-28T15:17:55Z 10000-01-01 2000 2000 The development of the nervous system involves a complex series of events that establish the intricate and precise neural network. Using the genetically manipulable Drosophila embryo and high resolution single cell analysis, I examined the function of commissureless, netrin, roundabout, and frazzled during neural development. Analysis of the individual axon pathways of RP3, V, and RP2 in wildtype and commissureless and roundabout mutants investigated how abnormal midline decisions affect subsequent growth cone pathfinding. Results showed that despite abnormal decisions at the midline, subsequent pathways and synaptic targeting in all cases were the perfect mirror-images of those seen in wildtype. This suggests that a growth cones' particular molecular experience and decision at the midline does not affects its ability to respond normally to subsequently presented cues. Further examination of the aCC, RP3, and RP2 motoneurons revealed that the midline mutants commissureless, netrin, frazzled and roundabout disrupt dendritic growth cone guidance at the CNS midline. Specifically, frazzled and roundabout act cell autonomously to mediate dendritic attraction and repulsion, respectively, at the midline supported by mutant analysis, cell autonomous rescue of function and protein localization to dendritic growth cones. In addition to its expression and function at the CNS midline, commissureless is also initially expressed on all muscle surfaces and then endocytosed during the period of neuro-musclar contact. Motoneurons fail to initiated synaptogenesis in commissureless mutants and resupplying wildtype commissureless back to commissureless null muscles restores motoneuron synaptogenesis. Misexpression of a cytoplasmically truncated commissureless construct in muscles functions as a dominant negative by inhibiting internalization and synaptogenesis. My findings support a role for commissureless modifying the molecular profile of the muscle surface allowing motoneuron synaptogenesis initiation. My research demonstrates that Drosophila midline mutants function not only in axon guidance but also in dendritic growth cone guidance and synaptogenesis initiation. Made available in DSpace on 2015-09-28T15:17:55Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9990190.pdf: 7668642 bytes, checksum: e399285b8f2792a875048293ecdf284b (MD5) Previous issue date: 2000 Embargo set by: Seth Robbins for item 88046 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 120 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2000. http://hdl.handle.net/2142/86765 (MiAaPQ)AAI9990190 eng Biology, Molecular Growth Cone Pathfinding and Neuromuscular Synaptogenesis in Drosophila Midline Mutants text Microbiology Microbiology University of Illinois at Urbana-Champaign Dissertation Ph.D.