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oai:www.ideals.illinois.edu:2142/86766 2023-07-11 col_2142_5131 col_2142_16338 com_2142_5130 com_2142_16337 com_2142_14793 com_2142_8903

Cronan, John E., Jr Campbell, John Walter 2015-09-28T15:17:56Z 2015-09-28T15:17:56Z 10000-01-01 2001 2001 The second ORF, b2323 is the fabB gene which produces beta-ketoacyl-ACP synthase I. This is one of two long chain specific condensing enzymes in E. coli and is required for unsaturated fatty acid biosynthesis. The other enzyme necessary for producing unsaturated fatty acids is 3-hydroxy-decanoyl-ACP dehydrase, encoded by the fabA gene. This gene has previously been shown to be positively regulated by FadR. Double fabA(Ts), fadR mutants have a synthetic lethal phenotype, fabB(Ts), fadR strains were constructed and shown to behave in a similar fashion. Experiments involving chloramphenicol acetyl transferase fusions confirm that FadR positively regulates transcription of fabB as well. Made available in DSpace on 2015-09-28T15:17:56Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9996620.pdf: 6132004 bytes, checksum: 6bd123ecc1e261271337812ee4aa3118 (MD5) Previous issue date: 2001 Embargo set by: Seth Robbins for item 88047 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 94 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2001. http://hdl.handle.net/2142/86766 (MiAaPQ)AAI9996620 eng Biology, Genetics Total Genomic Transcriptional Analysis of the Fatty Acid Degradation Regulon of Escherichia Coli text Microbiology Microbiology University of Illinois at Urbana-Champaign Dissertation Ph.D.