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oai:www.ideals.illinois.edu:2142/87067 2023-07-11 col_2142_5131 col_2142_16357 com_2142_5130 com_2142_16356 com_2142_8913 com_2142_8903

Bush, Daniel R. Lu, Mei-Yeh Jade 2015-09-28T15:23:00Z 2015-09-28T15:23:00Z 10000-01-01 1998 1998 "To explore other functionally-important domains, the AtSUC1 cDNA was randomly mutagenized and transformed yeast were screened on sucrose- or maltose-limited media for growth. Three point mutations were identified---H65Q, G334S, and L461P. Significantly, they all displayed enhanced sucrose transport rate. H65Q and G334S also increased maltose transport activity. In the predicted ""6-loop-6"" topology of the symporter, these residues all face extracellularly, suggesting their involvement in substrate binding. To investigate this hypothesis, the symporter-expressing COS-1 cells were analyzed by epitope-tagging and immunofluorescence. The results showed that both N- and C-termini, as well as the central loop, are located on the cytoplasmic side of the membrane." Made available in DSpace on 2015-09-28T15:23:00Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9912310.pdf: 3930939 bytes, checksum: f8e18bca2968d3dbadd6522ee8d7681a (MD5) Previous issue date: 1998 Embargo set by: Seth Robbins for item 88348 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 124 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1998. http://hdl.handle.net/2142/87067 (MiAaPQ)AAI9912310 eng Biology, Cell Molecular Cloning and Structure-Function Analysis of the Plant Proton-Sucrose Symporter text Plant Biology Plant Biology University of Illinois at Urbana-Champaign Dissertation Ph.D.