2026-09-21T11:51:48Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/87113 2023-07-11 col_2142_5131 col_2142_16405 com_2142_5130 com_2142_16404 com_2142_14833

Joanne B. Messick Berent, Linda Marie 2015-09-28T15:38:23Z 2015-09-28T15:38:23Z 10000-01-01 2002 2002 Little was known about the genome size or structure of the haemotrophic mycoplasmas and studies of pathogenicity, adhesion, and protein production are hampered by the inability to culture H. felis and use more common genetic tools. A renewable source of H. felis DNA in the form of a large insert library was created to study the genome and search for genes related to pathogenicity and survival. The genome size of H. felis was determined to be 1200 kb using pulsed field gel electrophoresis. A bacterial artificial chromosome (BAC) was used to create a large insert library of the H. felis genomic DNA. Using hybridization experiments and fingerprinting analysis, these BACs were ordered into a genomic encyclopedia with a restriction map. The BACs were then sub-cloned into a plasmid to generate high throughput genomic sequencing surveys (GSS) data. These GSSs were compared to the four completed sequenced mycoplasma and deposited in GenBank. This represents the first description of a physical and genomic map of an uncultivable mycoplasma. Made available in DSpace on 2015-09-28T15:38:23Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3044051.pdf: 5895277 bytes, checksum: dbe2eca1c0f37c956b6eaa0f0c07ffc4 (MD5) Previous issue date: 2002 Embargo set by: Seth Robbins for item 88394 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 124 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2002. http://hdl.handle.net/2142/87113 (MiAaPQ)AAI3044051 eng Biology, Genetics Haemobartonella Felis (Mycoplasma Haemofelis) Molecular Diagnostics and Genomic Studies text Veterinary Clinical Medicine Veterinary Clinical Medicine University of Illinois at Urbana-Champaign Dissertation Ph.D.