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oai:www.ideals.illinois.edu:2142/87212 2023-07-11 col_2142_5131 col_2142_16508 com_2142_5130 com_2142_16507 com_2142_14793 com_2142_8903

Gillette, Martha U. Artinian, Liana 2015-09-28T15:50:01Z 2015-09-28T15:50:01Z 10000-01-01 2001 2001 This study shows that stimulus-specific intracellular Ca2+ (Ca2+i) release controls the direction of clock resetting. Direct activation of the InsP3-induced Ca2+ i release (IICR) mimics cholinergic phase advance, while InsP3 , but not ryanodine, receptor antagonists block it. In contrast, selective activation of CICR with ryanodine results in glutamate-like delay. Ca 2+ imaging using multiphoton microscopy revealed signal-specific differences in Ca2+ transients. Cholinergic-stimulated Ca2+ transients are localized to the microdomains in the cytoplasm, whereas glutamate-induced Ca2+ transients are predominantly uniform across the cytosol and nucleus. Thus, the direction of clock resetting is determined by differential Ca2+i release that is likely to result in activation of spatially restricted Ca2+-dependent effector proteins. Made available in DSpace on 2015-09-28T15:50:01Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3017015.pdf: 6345951 bytes, checksum: 82fd26994e0c1aae16db53361f5f0f5b (MD5) Previous issue date: 2001 Embargo set by: Seth Robbins for item 88493 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 120 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2001. http://hdl.handle.net/2142/87212 (MiAaPQ)AAI3017015 eng Biology, Neuroscience Cholinergic Regulation of Circadian Rhythms in the SCN: Characterization of Signaling Mechanisms and the Role of Intracellular Calcium in the Directionality of Clock Resetting text Molecular and Integrative Physiology Molecular and Integrative Physiology University of Illinois at Urbana-Champaign Dissertation Ph.D.