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Nardulli, Ann M. Curtis-Ducey, Carol Dianne 2015-09-28T15:50:14Z 2015-09-28T15:50:14Z 10000-01-01 2009 2009 Interaction of estrogen receptor alpha (ERalpha) with 17beta-estradiol (E2) facilitates binding of the receptor to estrogen response elements (EREs) in target genes, which in turn leads to recruitment of coregulatory proteins. To better understand how estrogen-responsive genes are regulated, our laboratory identified a number of proteins that associate with the DNA-bound ERalpha. Our studies demonstrate that the nonmetastatic protein 23 homolog 1 (NM23-H1) interacts with ERalpha, increases ERalpha-ERE complex formation, influences ERalpha-mediated transcription and associates with the promoter region of the endogenous estrogen-responsive progesterone receptor (PR) gene. Furthermore, we show that a second protein, Apurinic/apyrimidinic endonuclease 1 or redox factor-1 (Ape1/Ref-1), interacts with ERalpha, promotes the ERalpha-ERE interaction, influences ERalpha-mediated transactivation, and selectively associates with endogenous, estrogen-responsive genes in MCF-7 cells. Our findings suggest that NM23-H1 and Ape1/Ref-1 are instrumental in modulating expression of estrogen-responsive genes. Interestingly, we demonstrate that Ape1/Ref-1 and NM23-H1, as well as the oxidative stress proteins Cu/Zn superoxide dismutase (SOD1), thioredoxin (Trx) and protein disulfide isomerase (PDI) are overexpressed in human breast cancer tissues. These studies provide a novel link between DNA repair, redox regulation and modulation of ERalpha-mediated transcription. Made available in DSpace on 2015-09-28T15:50:14Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3391919.pdf: 1204690 bytes, checksum: e8a801697c3f92719540610e8a73188a (MD5) Previous issue date: 2009 Embargo set by: Seth Robbins for item 88534 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 75 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2009. http://hdl.handle.net/2142/87253 (MiAaPQ)AAI3391919 eng Biology, Physiology DNA Repair, Redox Regulation and Modulation of Estrogen Receptor Alpha Mediated Transcription text University of Illinois at Urbana-Champaign Dissertation Ph.D. Molecular and Integrative Physiology Molecular and Integrative Physiology