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          <dc:date>2003</dc:date>
          <dc:date>2003</dc:date>
          <dc:contributor>Roberto Docampo</dc:contributor>
          <dc:creator>Rohloff, Peter John</dc:creator>
          <dc:description>Electron microscopic observations revealed a contractile vacuole coupled to acidocalcisomes in all three life cycle stages of the parasite. An aquaporin homologue localized to the acidocalcisomes and contractile vacuole. In epimastigotes expressing a GFP-aquaporin fusion protein, acidocalcisomes trafficked toward the contractile vacuole during hyposmotic stress in a cAMP- and microtubule-dependent fashion. This phenomenon was functionally significant, since aquaporin and microtubule inhibitors reduced the rate of volume recovery. Isolated contractile vacuoles were enriched in alkaline phosphatase, aquaporin, and some acidocalcisomal markers.</dc:description>
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Lift date: Forever
Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs</dc:description>
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          <dc:title>Osmoregulation in Trypanosoma Cruzi: The Role of the Acidocalcisome</dc:title>
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