2026-09-21T11:54:00Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/87872 2023-07-11 col_2142_5131 col_2142_13007 com_2142_5130 com_2142_13006

Sweedler, Jonathan V. Bora, Adriana 2015-09-28T21:56:36Z 2015-09-28T21:56:36Z 10000-01-01 2009 2009 The peptides with roles in the development of the pituitary were analyzed using transgenic mice. Hes1 KO is a genetically modified mouse that lives only e18.5 (embryonic days). Anterior pituitaries of Hes1 null mice exhibit hypoplasia due to increased cell death and reduced proliferation and in the intermediate lobe, the cells differentiate abnormally into somatotropes instead of melanotropes. These previous findings demonstrate that Hes1 has multiple roles in pituitary development, cell differentiation, and cell fate. AVP was detected in all samples. Interestingly, somatostatin [92-100] and provasopressin [151-168] were detected in the mutant but not in the wild type or heterozygous pituitaries while somatostatin-14 was detected only in the heterozygous pituitary. In addition, the putative peptide corresponding to m/z 1330.2 and POMC [205-222] are detected in the mutant and heterozygous pituitaries, but not in the wild type. These results indicate that Hes1 influences the processing of different prohormones having possible roles during development and opens new directions for further developmental studies. Made available in DSpace on 2015-09-28T21:56:36Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3406722.pdf: 58415666 bytes, checksum: f5d75509d8459004637b92f87ec3ac2e (MD5) Previous issue date: 2009 Embargo set by: Seth Robbins for item 89153 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 220 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2009. http://hdl.handle.net/2142/87872 (MiAaPQ)AAI3406722 eng Biology, Neuroscience Sampling Strategies for Characterization of Neuropeptides Using Mass Spectrometry and Functional Implications Into Cell -Cell Signaling text University of Illinois at Urbana-Champaign Dissertation Ph.D.