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oai:www.ideals.illinois.edu:2142/87928 2023-07-11 col_2142_5131 col_2142_14855 com_2142_5130 com_2142_14854 com_2142_14793 com_2142_8903

eng Biology, Cell Gillette, Martha U. Barnes, Jessica W. 2015-09-28T22:23:18Z 2015-09-28T22:23:18Z 10000-01-01 2004 2004 Research in our lab has determined that GLU activates Protein Kinase G (PKG), and that this activation is required for the mammalian GLU-induced phase advance. Since the Drosophila phase advance proceeds through phosphorylation-dependent degradation of Drosophila TIMELESS (dTIM), I hypothesized that the mammalian light-/GLU-induced phase advance proceeds through PKG-dependent phosphorylation of mammalian TIMELESS (mTIM). In the course of testing this hypothesis, we discovered two isoforms of mTIM, one encoding the full length protein (mTIM-fl) and the other a short splice variant (mTIM-s) corresponding to the last third of mTIM-fl. Only mTIM-fl oscillates in the SCN, associates with other core clock elements, is necessary for circadian rhythmicity, and is degraded by light and GLU in the late night. These findings have contributed to placing mTIM within the core molecular clockwork of mammals, and established both the target of the phase advance signaling pathway and the mechanism by which it is regulated. Made available in DSpace on 2015-09-28T22:23:18Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3130879.pdf: 5764026 bytes, checksum: e60e4b619560edc0812c7d28ba911663 (MD5) Previous issue date: 2004 Embargo set by: Seth Robbins for item 89209 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 85 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2004. http://hdl.handle.net/2142/87928 (MiAaPQ)AAI3130879 Mammalian TIMELESS and Protein Kinase G: Partners Linking the Molecular Clockwork to Environmental Light text University of Illinois at Urbana-Champaign Dissertation Ph.D.