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Cheeseman, John M. Lexa, Matej 2015-09-28T22:23:47Z 2015-09-28T22:23:47Z 10000-01-01 1999 1999 In this dissertation, I have explored this question experimentally. My approach was to obtain plants with NO3- reduction mostly in the roots or mostly in the leaves, measure growth and biomass allocation parameters of those plants when grown on NO3-, and compare them to growth of wild-type (WT) plants and nitrate reductase deficient mutants. Two techniques were used to manipulate the site of NO 3- reduction: (i) grafting and (ii) genetic transformation. Grafted wildtype and nitrate reductase-deficient pea plants adapted to changes in the location of NO3- reduction. The results support the idea of rapid mixing of metabolite pools and provide evidence for shoot to root signalling of nitrogen status. Utilizing tissue-specific promoters, plants were created with altered levels of nitrate reductase in roots and shoots. The results showed that transformation of WT plants with nia2 tobacco gene resulted in greater alterations in NRA than the transformation of the corresponding double mutant. Changes in growth and co-suppression-like leaf chlorosis was the most frequent outcome, often causing a lowering or disappearance of NRA. A negative correlation between the ratio of root and shoot reduction of nitrate and the root shoot ratio of transformed plants was found. The possible reasons for differences between the two approaches are discussed. Made available in DSpace on 2015-09-28T22:23:47Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9921710.pdf: 7236432 bytes, checksum: 2bfc301e098459875ca13e8bd02a09d4 (MD5) Previous issue date: 1999 Embargo set by: Seth Robbins for item 89213 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs U of I Only 173 p. Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1999. http://hdl.handle.net/2142/87932 (MiAaPQ)AAI9921710 eng Agriculture, Agronomy The Effect of the Site of Nitrogen Assimilation on Plant Growth text University of Illinois at Urbana-Champaign Dissertation Ph.D.