2026-09-20T22:16:59Z https://www.ideals.illinois.edu/oai-pmh

oai:www.ideals.illinois.edu:2142/87973 2023-07-11 col_2142_5131 col_2142_14789 com_2142_5130 com_2142_14788 com_2142_8903

Bailey, Ryan C. Bailey, Ryan C. Harley, Brendan A. Murphy, Catherine J. Perry, Richard H. Turgeon, Aurora 2015-09-29T20:37:47Z 2015-09-29T20:37:47Z 2015-08 2015-07-06 The extracellular matrix is a complex and dynamic collection of collagens, non-collagenous matrix proteins, glycosaminoglycans and growth factors. The ECM plays a significant role in guiding the behavior of cells in their tissue specific niche. The recapitulation of both the mechanical and biochemical cues of the ECM is an important objective towards in vitro engineering of functional tissue replacements. Herein I describe my efforts towards the biochemical modification of 3D, porous collagen scaffolds as ECM mimics. Benzophenone mediated photochemistry was used to decorate scaffolds with patterns of vascular endothelial growth factor, which were shown to induce endothelial cells towards a vasculogenic lineage. The use of a two photon excitation for BP patterning was proven affective for surface patterning but ineffective for patterning past a couple hundreds of microns. Heterogenous scaffold patterning in all three dimensions was achieved by collagen slurry layering prior to scaffold freeze drying. A single scaffold was created with two distinct compartment a high modulus “bone” compartment and a low modulus “cartilage” compartment. . This technique was used to model the osteochondral interface. Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2015-09-29 without embargo terms The student, Aurora Turgeon, accepted the attached license on 2015-06-19 at 15:57. The student, Aurora Turgeon, submitted this Dissertation for approval on 2015-06-19 at 16:04. This Dissertation was approved for publication on 2015-07-06 at 09:17. DSpace SAF Submission Ingestion Package generated from Vireo submission #8297 on 2015-09-29 at 13:21:35 Made available in DSpace on 2015-09-29T20:37:47Z (GMT). No. of bitstreams: 2 TURGEON-DISSERTATION-2015.pdf: 3945890 bytes, checksum: 093532c6ca9e0143784411f0430ebcf3 (MD5) LICENSE.txt: 4211 bytes, checksum: 215d6c6267600cc0978f66eb96ce455c (MD5) Previous issue date: 2015-07-06 application/pdf http://hdl.handle.net/2142/87973 en Copyright 2015 Aurora Turgeon Photopatterning benzophenone scaffold The heterogeneous biochemical modification of porous collagen scaffolds for tissue engineering applications text text 2015-8 Chemistry Chemistry University of Illinois at Urbana-Champaign Dissertation Ph.D.