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Boppart, Stephen A. Ark, Eugene D 2016-03-02T21:06:42Z 2016-03-02T21:06:42Z 2018-03-03T10:15:28Z 2015-11-24 2015-12 Optical techniques have garnered increasing interest in biomedical research. As the types of optical probes available continue to expand, the appeal of experiments utilizing multiple probes simultaneously grows rapidly. Unfortunately, the significant spectral overlap in probe responsiveness presents limitations on their potential applications. Much of the effort in circumventing this issue has been focused on developing modified probes with shifted spectral sensitivities; however, this is a long and inefficient process that tends to have undesired consequences on probe activity. This thesis explores the possibility of using temporal pulse shaping in order to improve the selectivity with which optically sensitive molecules can be activated. It is demonstrated that linearly chirped pulses and dark pulses have the potential to improve selectivity by exclusively controlling the spectral phase function of the illumination source for three classes of optical probes: fluorescent molecules, caged compounds, and opsins. Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2017-12-01 The student, Eugene Ark, accepted the attached license on 2015-11-24 at 15:28. The student, Eugene Ark, submitted this Thesis for approval on 2015-11-24 at 15:32. This Thesis was approved for publication on 2015-11-24 at 16:14. DSpace SAF Submission Ingestion Package generated from Vireo submission #8839 on 2016-03-02 at 14:13:24 Made available in DSpace on 2016-03-02T21:06:42Z (GMT). No. of bitstreams: 2 ARK-THESIS-2015.pdf: 8016939 bytes, checksum: b06ca23f3efcb033471674898e0d83ee (MD5) LICENSE.txt: 4207 bytes, checksum: 4b39c8588764aaef9443cc83ea6a2429 (MD5) Previous issue date: 2015-11-24 Embargo set by: Seth Robbins for item 91412 Lift date: 2018-03-02T21:07:27Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system Limited Restriction Lifted for Item 91412 on 2018-03-03T10:15:28Z. application/pdf http://hdl.handle.net/2142/89209 en Copyright 2015 Eugene D. Ark Femtosecond pulse shaping caged compound opsin Femtosecond pulse shaping for enhanced optical imaging and control of cells text text Electrical & Computer Engineering Electrical & Computer Engineering University of Illinois at Urbana-Champaign Thesis M.S.